Journal: PLOS ONE

Article Title: Monocyte-cancer cell fusion is mediated by phosphatidylserine—CD36 receptor interaction and induced by ionizing radiation

doi: 10.1371/journal.pone.0311027

Figure Lengend Snippet: Fluorescent microscopy of CellTrace and CD36 staining A) MCF-7 cells stained with CellTrace Far red B) THP-1 cells stained with CellTrace Violet C) THP-1 cells stained with CD36 antibodies. All images were taken with x40 magnification.

Article Snippet: The cell cultures were washed in PBS, incubated with Human TruStain FcX TM (Fc Receptor Blocking Solution) (BioLegend, 422302, San Diego, USA) for 10 minutes at 4°C, 1:20 ratio to Cell Staining Buffer (Nordic Biosite, ASB-022501L, Täby, Sweden), followed by staining with 5 μg/ml Anti-Human CD36/SCARB3 Antibody (Phycoerythrin (PE)-conjugated) (Rabbit monoclonal IgG antibody, Sino Biological, 10752-R001-P, Beijing, China), or 50 μg/ml Rabbit IgG Isotype Control, PE-conjugated (Bioss, bs-0295P-PE, Massachusetts, USA) for 30 minutes in darkness at 4°C.

Techniques: Microscopy, Staining

Journal: PLOS ONE

Article Title: Monocyte-cancer cell fusion is mediated by phosphatidylserine—CD36 receptor interaction and induced by ionizing radiation

doi: 10.1371/journal.pone.0311027

Figure Lengend Snippet: Panel A shows THP-1 and MCF-7 cells cultured in separate flasks and treated with 0, 2.5, 5, and 10 Gy radiation. After that, the cells were analyzed by flow cytometry to detect the proportion of cells expressing PS and CD36. Panel B shows a cell fusion experiment where THP-1 cells (labelled with CellTrace Violet) and MCF-7 cells (labelled with CellTracer Far Red) are grown in the same flask and treated with 0 and 5 Gy gamma radiation. After 2 days of co-culture, the cells are sorted with FACS Aria III. Cells co-expressing CellTrace Violet and Far Red are assessed as THP1-/MCF-5 tumor hybrid cells.

Article Snippet: The cell cultures were washed in PBS, incubated with Human TruStain FcX TM (Fc Receptor Blocking Solution) (BioLegend, 422302, San Diego, USA) for 10 minutes at 4°C, 1:20 ratio to Cell Staining Buffer (Nordic Biosite, ASB-022501L, Täby, Sweden), followed by staining with 5 μg/ml Anti-Human CD36/SCARB3 Antibody (Phycoerythrin (PE)-conjugated) (Rabbit monoclonal IgG antibody, Sino Biological, 10752-R001-P, Beijing, China), or 50 μg/ml Rabbit IgG Isotype Control, PE-conjugated (Bioss, bs-0295P-PE, Massachusetts, USA) for 30 minutes in darkness at 4°C.

Techniques: Cell Culture, Flow Cytometry, Expressing, Co-Culture Assay

Journal: PLOS ONE

Article Title: Monocyte-cancer cell fusion is mediated by phosphatidylserine—CD36 receptor interaction and induced by ionizing radiation

doi: 10.1371/journal.pone.0311027

Figure Lengend Snippet: The proportion of MCF-7 expressing PS increases in dose-dependent relation (C). No correlation of PS and CD36 expression in THP-1 cells nor CD36 expression in MCF-7 cells was found in relation to the dose of ionizing radiation.

Article Snippet: The cell cultures were washed in PBS, incubated with Human TruStain FcX TM (Fc Receptor Blocking Solution) (BioLegend, 422302, San Diego, USA) for 10 minutes at 4°C, 1:20 ratio to Cell Staining Buffer (Nordic Biosite, ASB-022501L, Täby, Sweden), followed by staining with 5 μg/ml Anti-Human CD36/SCARB3 Antibody (Phycoerythrin (PE)-conjugated) (Rabbit monoclonal IgG antibody, Sino Biological, 10752-R001-P, Beijing, China), or 50 μg/ml Rabbit IgG Isotype Control, PE-conjugated (Bioss, bs-0295P-PE, Massachusetts, USA) for 30 minutes in darkness at 4°C.

Techniques: Expressing

Journal: PLOS ONE

Article Title: Monocyte-cancer cell fusion is mediated by phosphatidylserine—CD36 receptor interaction and induced by ionizing radiation

doi: 10.1371/journal.pone.0311027

Figure Lengend Snippet: The proportion of THP-1/MCF-7 tumor hybrid cells in co-cultures supplemented with 40 μg/ml and 80 μg/ml CD36 antibody was 1.8% and 1.9%, respectively. In non-treated THP-1/MCF-7 co-cultures, the proportion of tumor hybrid cells (3.6%) was significantly higher compared to co-cultures treated with anti-CD36 antibody (P<0.001).

Article Snippet: The cell cultures were washed in PBS, incubated with Human TruStain FcX TM (Fc Receptor Blocking Solution) (BioLegend, 422302, San Diego, USA) for 10 minutes at 4°C, 1:20 ratio to Cell Staining Buffer (Nordic Biosite, ASB-022501L, Täby, Sweden), followed by staining with 5 μg/ml Anti-Human CD36/SCARB3 Antibody (Phycoerythrin (PE)-conjugated) (Rabbit monoclonal IgG antibody, Sino Biological, 10752-R001-P, Beijing, China), or 50 μg/ml Rabbit IgG Isotype Control, PE-conjugated (Bioss, bs-0295P-PE, Massachusetts, USA) for 30 minutes in darkness at 4°C.

Techniques: